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Breaks actimeter2/12/2024 ![]() Practical considerations, such as relative cost versus degree of versatility, should be considered when deciding which system to use for the screening of test compounds during the earliest stages of preclinical drug development.Ĭopyright © 2011 Elsevier Inc. Infrared beam-based activity detection systems and LABORAS provide relatively-comparable quantitative data regarding locomotor activity. Rats received saline or Asenapine (0.01 and 0.03 mg/kg, s.c.) 30 min before the end of REM SD, and just after the 72 hours of REM SD, they were tested for locomotor activity in Plexiglas cages (0.41×0.26×0.20 m) placed in an actimeter (Imetronic, France) endowed with infrared beams positioned 0.04 m and 0.12 m above the floor. ![]() Motor behavior assessment using Actimeter and. The two automated activity-detection systems exhibited similar sensitivities in determining changes in locomotor activity, but with the LABORAS being more sensitive than the Actimeter in detecting caffeine-induced increases in vertical activity (rearing behavior). During the test, horizontal locomotor activity (movements) was determined by breaks in movement-sensitive photobeams that were then converted into locomotor activity counts, and vertical activity was recorded as the number of rearing episodes breaking the photocell beams of the upper frame. Firstly, it is noticeable that all the incoming data from the actimeter have a slight correlation with the diary notations. Acidified drinking water in Cln2 R207X mice, in comparison to WT mice, changed several locomotor and behavioral parameters as measured in the force-plate actimeter: the area covered and total. In all three assays, dose-dependent sedation- and excitation-related effects were observed with chlorpromazine and caffeine, respectively. Figure 2a presents the correlation matrix between the variables collected with the actimeter, the calculated features, and the binary series (off-wrist 1, on-wrist 0) derived from participants’ self-reports in the diary. A modified Irwin test was also performed to visually observe the neurobehavioral effects. Spontaneous locomotor activity counts obtained from two automated test systems - an infrared beam-based activity meter (Actimeter) and the mechanical vibration-based LABORAS - were compared in rats dosed with chlorpromazine (2-8mg/kg) or caffeine (3-24mg/kg), p.o. Force-Plate Actimeter (FPA)-based assessments can quantify locomotor activity and detailed motor activity with an incredibly rich data stream that can reveal details of movement unobservable by the naked eye. However, at earlier stages, when a greater number of test compounds must be screened for potential CNS side effects, locomotor activity assessment may be a better tool for the comparison of compounds. General neurobehavioral assays, like a modified Irwin test or a functional observational battery, are necessary for central nervous system (CNS) safety pharmacology testing near the end of the target validation (early discovery) stage of preclinical drug development.
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